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hybridization protocol, microarrays  (Operon Technologies Inc)

 
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    Operon Technologies Inc hybridization protocol, microarrays
    Hybridization Protocol, Microarrays, supplied by Operon Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hybridization+protocol%2C+microarrays/hybridization+protocol++microarrays/us07145655-232-5-25
    Average 90 stars, based on 1 article reviews
    hybridization protocol, microarrays - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Hybridization:

    Article Title: Methods and apparatus for measuring refractive index and optical absorption differences
    Article Snippet: Following the hybridization step, the array was washed in 2×SSC, 0.2% SDS for approximately 1 minute, then in 0.2×SSC, and then in 0.05×SSC, following the Operon Technologies, Inc. “Hybridization Protocol, Microarrays” (2001) protocol to remove non-hybridized cDNA.

    Article Title: Methods and apparatus for measuring refractive index and optical absorption differences
    Article Snippet: Following the hybridization step, the array was washed in 2×SSC, 0.2%SDS for approximately 1 minute, then in 0.2×SSC, and then in 0.05×SSC, following the Operon Technologies, Inc. “Hybridization Protocol, Microarrays” (2001) protocol to remove non-hybridized cDNA.

    Article Title: Methods and apparatus for measuring refractive index and optical absorption differences
    Article Snippet: The denatured, Cy5-labeled cDNA molecules were then allowed to hybridize with the array on the slides at 67° C. for 12 hours or overnight under standard conditions set forth in the Operon Technologies, Inc. “Hybridization Protocol, Microarrays” (2001) protocol, incorporated herein by reference.

    Reverse Transcription:

    Article Title: Methods and apparatus for measuring refractive index and optical absorption differences
    Article Snippet: Following the hybridization step, the array was washed in 2×SSC, 0.2% SDS for approximately 1 minute, then in 0.2×SSC, and then in 0.05×SSC, following the Operon Technologies, Inc. “Hybridization Protocol, Microarrays” (2001) protocol to remove non-hybridized cDNA.

    Article Title: Methods and apparatus for measuring refractive index and optical absorption differences
    Article Snippet: Following the hybridization step, the array was washed in 2×SSC, 0.2%SDS for approximately 1 minute, then in 0.2×SSC, and then in 0.05×SSC, following the Operon Technologies, Inc. “Hybridization Protocol, Microarrays” (2001) protocol to remove non-hybridized cDNA.

    Article Title: Methods and apparatus for measuring refractive index and optical absorption differences
    Article Snippet: The denatured, Cy5-labeled cDNA molecules were then allowed to hybridize with the array on the slides at 67° C. for 12 hours or overnight under standard conditions set forth in the Operon Technologies, Inc. “Hybridization Protocol, Microarrays” (2001) protocol, incorporated herein by reference.

    Isolation:

    Article Title: Methods and apparatus for measuring refractive index and optical absorption differences
    Article Snippet: Following the hybridization step, the array was washed in 2×SSC, 0.2% SDS for approximately 1 minute, then in 0.2×SSC, and then in 0.05×SSC, following the Operon Technologies, Inc. “Hybridization Protocol, Microarrays” (2001) protocol to remove non-hybridized cDNA.

    Article Title: Methods and apparatus for measuring refractive index and optical absorption differences
    Article Snippet: Following the hybridization step, the array was washed in 2×SSC, 0.2%SDS for approximately 1 minute, then in 0.2×SSC, and then in 0.05×SSC, following the Operon Technologies, Inc. “Hybridization Protocol, Microarrays” (2001) protocol to remove non-hybridized cDNA.

    Article Title: Methods and apparatus for measuring refractive index and optical absorption differences
    Article Snippet: The denatured, Cy5-labeled cDNA molecules were then allowed to hybridize with the array on the slides at 67° C. for 12 hours or overnight under standard conditions set forth in the Operon Technologies, Inc. “Hybridization Protocol, Microarrays” (2001) protocol, incorporated herein by reference.



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    Image Search Results


    (A) Control and IFIT1-depleted THP1 cells were stimulated with 100 ng/mL LPS for 1, 2, 4, 6, and 24 hr, and transcriptional responses were measured by microarray. The upper heatmap shows genes with substantial expression increases in WT cells compared to their expression level in IFIT1-depleted cells. The lower heatmap shows the sum of the expression difference between WT and IFIT1-depleted cells across the time course . (B and C) The network connections of genes that showed (B) enhanced expression (group 1; ) or (C) reduced expression (group 2; ) in IFIT1-depleted cells, as determined by IPA. (D) Transfection of siRNA into IFIT1 shRNA-expressing cells sustains a stable suppression of IFIT1 induction in response to 100 ng/mL LPS. (E and F) Control and IFIT1-depleted THP1 cells (E) or human primary macrophages (F) were stimulated with 100 ng/mL LPS, and secreted IFN-β protein was measured by ELISA. Data are representative of two (A) or three (D–F) independent experiments. Data in (D)–(F) are expressed as mean SD; *p < 0.05, **p < 0.01 (two-way ANOVA followed by Sidak’s multiple comparison test). See also and , , and .

    Journal: Cell reports

    Article Title: IFIT1 Exerts Opposing Regulatory Effects on the Inflammatory and Interferon Gene Programs in LPS-Activated Human Macrophages

    doi: 10.1016/j.celrep.2018.09.002

    Figure Lengend Snippet: (A) Control and IFIT1-depleted THP1 cells were stimulated with 100 ng/mL LPS for 1, 2, 4, 6, and 24 hr, and transcriptional responses were measured by microarray. The upper heatmap shows genes with substantial expression increases in WT cells compared to their expression level in IFIT1-depleted cells. The lower heatmap shows the sum of the expression difference between WT and IFIT1-depleted cells across the time course . (B and C) The network connections of genes that showed (B) enhanced expression (group 1; ) or (C) reduced expression (group 2; ) in IFIT1-depleted cells, as determined by IPA. (D) Transfection of siRNA into IFIT1 shRNA-expressing cells sustains a stable suppression of IFIT1 induction in response to 100 ng/mL LPS. (E and F) Control and IFIT1-depleted THP1 cells (E) or human primary macrophages (F) were stimulated with 100 ng/mL LPS, and secreted IFN-β protein was measured by ELISA. Data are representative of two (A) or three (D–F) independent experiments. Data in (D)–(F) are expressed as mean SD; *p < 0.05, **p < 0.01 (two-way ANOVA followed by Sidak’s multiple comparison test). See also and , , and .

    Article Snippet: Each condition was represented by two biological replicates. cRNA amplification and labeling were performed using the Illumina TotalPrep RNA Amplification Kit (Ambion), microarray hybridization and scanning protocols followed standard Illumina protocols.

    Techniques: Microarray, Expressing, Transfection, shRNA, Enzyme-linked Immunosorbent Assay

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: IFIT1 Exerts Opposing Regulatory Effects on the Inflammatory and Interferon Gene Programs in LPS-Activated Human Macrophages

    doi: 10.1016/j.celrep.2018.09.002

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Each condition was represented by two biological replicates. cRNA amplification and labeling were performed using the Illumina TotalPrep RNA Amplification Kit (Ambion), microarray hybridization and scanning protocols followed standard Illumina protocols.

    Techniques: Recombinant, Microarray, shRNA, Software